課程名稱 |
生物能量與代謝工程 Bioenergetics and Metabolic Engineering |
開課學期 |
105-2 |
授課對象 |
電機資訊學院 生醫電子與資訊學研究所 |
授課教師 |
魏安祺 |
課號 |
BEBI5012 |
課程識別碼 |
945 U3020 |
班次 |
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學分 |
3.0 |
全/半年 |
半年 |
必/選修 |
選修 |
上課時間 |
星期二2,3,4(9:10~12:10) |
上課地點 |
博理212 |
備註 |
總人數上限:30人 |
Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/1052BEBI5012 |
課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
課程大綱
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課程概述 |
Metabolic engineering focus on using a systems-level view to analyze cellular metabolism and predict optimal rewiring of metabolic networks. It integrates large-scale data collection, high-throughput biology and in silico modeling along with genome-wide engineering to improve cellular phenotype or pathway flux.
The first half of the class will review and consolidate concepts in bioenergetics, focusing on bioenergetics principles, mitochondria physiology and pathology, and metabolism pathways. The second half of the class will introduce metabolic engineering and its applications. Topics will include integration of the techniques of molecular biology with the tools of mathematical analysis, to help elucidate metabolic flux control and rational selection of targets for genetic modification.
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課程目標 |
The class will introduce basic principles of bioenergetics, reviewing cellular metabolic pathways, and connecting the area of systems biology with metabolic engineering. The objective of the class is providing a quantitative basis for the understanding of metabolic networks from single cell to organ level. Students will also be expected to read recent papers in the related field, investigate one topic and make a short class presentation. |
課程要求 |
普通生物學,微積分 |
預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
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參考書目 |
1. Bioenergetics 4, (2013), by David Nicholls
2. Systems Biology: Constraint-based Reconstruction and Analysis, 2nd
ed. Edition (2016) by Bernhard Ø. Palsson
3. Metabolic Engineering: Principles and Methodologies (1998) by
Gregory N. Stephanopoulos
4. Lehninger Principle of Biochemistry
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評量方式 (僅供參考) |
No. |
項目 |
百分比 |
說明 |
1. |
期中考 |
25% |
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2. |
期末考 |
25% |
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3. |
期末報告 |
30% |
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4. |
課堂參與 |
20% |
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週次 |
日期 |
單元主題 |
第1週 |
2/21 |
Introduction of Bioenergetics
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第2週 |
2/28 |
No class |
第3週 |
3/07 |
Chemiosmotic theory
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第4週 |
3/14 |
Energy transduction, Oxidative phosphorylation |
第5週 |
3/21 |
Respiratory chain
Cellular bioenergetics |
第6週 |
3/28 |
Mitochondria physiology & pathophysiology
Review/Discussion |
第7週 |
4/04 |
No class |
第8週 |
4/11 |
Midterm exam |
第9週 |
4/18 |
Introduction of cellular metabolism
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第10週 |
4/25 |
Biochemical reaction networks and metabolic network reconstruction
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第11週 |
5/02 |
Mathematical properties of reconstructed networks
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第12週 |
5/09 |
Mathematical properties of reconstructed networks
Metabolic flux analysis
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第13週 |
5/16 |
PartIII: (ch 15-21) Determining the phenotypic potential of reconstructed Network
Metabolic flux analysis (COBRA toolbox) |
第14週 |
5/23 |
Omic data integration and analysis
Applications of metabolic engineering
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第15週 |
5/30 |
No Class |
第16週 |
6/06 |
Oral presentation I
6/8 1:00-2:20 電二104 seminar "Super-Resolution 3D Molecular Tracking Microscopes and Silver Nanocluster-Based Biosensors" |
第17週 |
6/13 |
Oral presentation II
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第18週 |
6/20 |
Final Exam |
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